Purification and characterization of a tuliposide-converting enzyme from bulbs of Tulipa gesneriana.
نویسندگان
چکیده
An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral pH and moderate temperature. The enzyme preferentially acted on such glucose esters as 6-tuliposides, and to a lesser extent on p-nitrophenylacetate.
منابع مشابه
A novel lactone-forming carboxylesterase: molecular identification of a tuliposide A-converting enzyme in tulip.
Tuliposides, the glucose esters of 4-hydroxy-2-methylenebutanoate and 3,4-dihydroxy-2-methylenebutanoate, are major secondary metabolites in tulip (Tulipa gesneriana). Their lactonized aglycons, tulipalins, function as defensive chemicals due to their biological activities. We recently found that tuliposide-converting enzyme (TCE) purified from tulip bulbs catalyzed the conversion of tuliposide...
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ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 73 8 شماره
صفحات -
تاریخ انتشار 2009